Molecular Formula | C12H13NO4 |
Molar Mass | 235.24 |
Density | 1.30±0.1 g/cm3(Predicted) |
Melting Point | 114 °C |
Boling Point | 365.0±48.0 °C(Predicted) |
Solubility | Soluble in DMSO. |
Appearance | Light yellow crystalline solid. |
Color | Off-white |
pKa | 9.22±0.30(Predicted) |
Storage Condition | Keep in dark place,Sealed in dry,Store in freezer, under -20°C |
Stability | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 2 months. |
Sensitive | Light Sensitive |
In vitro study | ISO-1 significantly inhibited cytokine activity in vitro. It inhibits the release of tumor necrosis factor from macrophages isolated from LPS-treated wild-type mice, but has no effect on the release of cytokines from MIF-deficient macrophages. |
In vivo study | For severe sepsis caused by endotoxin or cecal ligation and puncture, ISO-1 treatment significantly increased survival. In a mouse asthma model established with OVA, airway hyperresponsiveness was prevented by ISO-1. ISO-1 attenuates ozone-induced cell recruitment, cytokine release, and airway hyperresponsiveness, but does not affect treatment of emphysema. |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 4.251 ml | 21.255 ml | 42.51 ml |
5 mM | 0.85 ml | 4.251 ml | 8.502 ml |
10 mM | 0.425 ml | 2.125 ml | 4.251 ml |
5 mM | 0.085 ml | 0.425 ml | 0.85 ml |
biological activity | ISO-1 (MIF Antagonist) is an inhibitor of MIF (macrophage migration inhibitory factor) D-isomerase activity, IC50 is 7 μM. |
target | TargetValue MIF () 7 μM |
Target | Value |
MIF () | 7 μM |
in vitro study | ISO-1 significantly inhibited cytokine activity in vitro. It inhibits the release of tumor necrosis factor from macrophages (isolated from LPS-treated wild-type mice), but has no effect on cytokines released by MIF-deficient macrophages. |
in vivo study | for severe sepsis caused by endotoxin or cecal ligation and perforation, ISO-1 treatment can significantly increase survival rate. In the mouse asthma model established by OVA, ISO-1 can prevent airway hyperresponsiveness. ISO-1 attenuates ozone-induced cell recruitment, cytokine release and airway hyperresponsiveness, but does not affect the treatment of emphysema. |